منابع مشابه
Quantitative assay for algal chemotaxis.
A quantitative capillary assay is described for measuring chemoreception in the neritic and littoral unicellular alga Dunaliella tertiolecta. Lucite chemotaxis plates were used in the assay with 3-microliter capillaries. A Coulter Counter was employed to determine algal cell numbers. D. tertiolecta is attracted to ammonium ion with a maximum positive response at 10(-3) M. Inclusion of calcium a...
متن کاملQuantitative and dynamic assay of single cell chemotaxis.
We have developed a single-cell assay platform that allows quantitative analysis of single cell chemotaxis by dynamic morphogenetic gradients, subcellular microscopic imaging and automated image analysis, and have applied these to measure cellular polarization of budding yeast. The computer-controlled microfluidic device regulates the gradient profile at any given time, and allows quantitative ...
متن کاملC. elegans chemotaxis assay.
Many organisms use chemotaxis to seek out food sources, avoid noxious substances, and find mates. Caenorhabditis elegans has impressive chemotaxis behavior. The premise behind testing the response of the worms to an odorant is to place them in an area and observe the movement evoked in response to an odorant. Even with the many available assays, optimizing worm starting location relative to bot...
متن کاملAn Algal Assay Technique for Aquatic Toxicants
A technique was developed for algal toxicity assays. Algal communities obtained from several sewage treatment plants and surface waters were the test specimens. The toxicant used was zinc acetate The techniques that were developed are relatively simple, rapid, and precise. Algal responses to toxicants were measured by changes in oxygen production, and two types of responses were observed. The v...
متن کاملA real-time assay for neutrophil chemotaxis.
Neutrophils are the predominant cells during acute phases of inflammation, and it is now recognized that these leukocytes play an important role in modulation of the immune response. Directed migration of these cells to the sites of injury, known as chemotaxis, is driven by chemoattractants present at the endothelial cell surface or in the extracellular matrix (ECM). Since uncontrolled or exces...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1978
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.36.6.847-850.1978